pharmcal: HPLC Mobile Phase (Isocratic)

HPLC Mobile Phase Calculator (Isocratic) | PharmCal Professional

HPLC Mobile Phase (Isocratic)

Calculate total solvent volume based on Run Time and Overage

INPUTS

Flow Rate

mL/min

Runtime

min

No. of Injections

Inj.

Required Overage

OUTPUT

Minimum Volume Required: 0 mL

Volume to Prepare (w/ Overage): 0 mL

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How to Use the HPLC Mobile Phase Calculator

Proper planning of mobile phase preparation is essential in High-Performance Liquid Chromatography (HPLC) to prevent running dry during a sequence, which can damage the column and pump seals.

Step 1: Enter Method Parameters

Input the Flow Rate (mL/min) and the total Runtime (minutes per injection) as defined in your analytical method (STP).

Step 2: Enter Sequence Details

Enter the total Number of Injections. This should include: System Suitability, Standards, Samples, and Bracketing Standards.

Step 3: Add Safety Overage

It is GMP best practice to prepare excess mobile phase to account for evaporation, priming, and potential re-injections. A standard overage is between 10% to 20%.

Key Calculation Formula

Total Vol = (Flow × Time × Inj) × (1 + %Overage/100)

Labels: HPLC Mobile Phase (Isocratic)